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KMID : 0357419950250020213
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Korean journal of Virology
1995 Volume.25 No. 2 p.213 ~ p.222
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Effects of Virus Infection Ratio on Cell Attachment and Virus Titer in Microcarrier Culture
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전복환
박호선/우규진/황규계/박송용/문홍모
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Abstract
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We had previously evaluated the possibility of using microcarrier culture system to produce attenuated cell-associated and cell-free varicella-zoster virus (VZV). We have investigated the attachment characteristics of both normal human embryonic
lung(LuMA) cells and virus-infected LuMA cells on cell-grown microcarriers with various multiplicities of infection. In cell at tachment period for virus infection, a portion of the unattached normal cells in culture medium was increased with the
decrease of unattached VZV-infected cells regardless of infection ratio. VZV yield was enhanced with increase of initial virus-infected cells onto cell-laden microcarriers. By understanding relationship between the attachment kinetics of cells
and
virus
titers and by determining virus infection ratio, it might be possible to propagate the VZV efficiently using microcarrier culture system.
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KEYWORD
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